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Transcriptional profiling of Xanthomonas oryzae pv. oryzae

Identification of sux cluster in Xoo by RNAseq

PXO99A cells were grown in NB media with and without supplementation of sugar and were sampled over eight hours for an in vitro RNAseq analysis. A) The first two principal components explaining the variance between Xoo grown in NB (grey), NB + sucrose (pink), and NB + glucose (blue). B) RNAseq data of Xoo grown for 4 hours in the three media types, where the abundance of each gene is assigned radial coordinates and color based on which treatment has more abundant RNA. The z-axis is the –log10 p-value with a significant p < 0.01. C) Proposed model of the sux cluster-encoded sucrose uptake system in Xoo, including a schematic representation of the gene cluster. SuxA (brown): β-barrel outer membrane porin; SuxC (blue): major facilitator superfamily (MFS)-type sucrose/H+-symporter; SuxB (pink): cytosolic sucrose hydrolase; SuxR (purple): LacI-type transcriptional repressor. D) RNAseq read counts for suxC (blue), suxA (brown), and suxB (pink) after 6 hours of growth in media without additional sugar (Ctr) or supplemented with 30 mM sucrose (Suc). Values were normalized to the 0 h time point and the condition without additional sugar.

Interactive 3D Volcano Plot

References

Zöllner N., Juying Long J., Congfeng Song S., Sharkey J., Sadoine M., Loo E.P.I., Yang B. & Frommer W.B. (2025) The role of sux cistron-mediated sucrose uptake for virulence of the rice blight pathogen Xanthomonas oryzae pv. oryzae. biorXiv. PNAS Nexus in revision.