Sensors for Plant Sciences

Compiled by Yuuma Ishikawa, Mayuri Sadoine & Wolf B. Frommer

These lists were compiled for an “update” section for Plant Physiology (Sadoine et al., 2021). Please contact us if you would like to add information or add comments.  

 

Table 1. List of ion and metabolite and transporter sensors for plant sciences*

* extensive list, but far from being comprehensive

m, mutant; smMLCK, smooth-muscle myosin light-chain kinase; p, peptide

 

Table 2. Calcium sensors

References

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Ahmad M, Mohsin M, Iqrar S, Manzoor O, Siddiqi TO, Ahmad A (2018) Live cell imaging of vitamin B12 dynamics by genetically encoded fluorescent nanosensor. Sens Actuators B Chem 257: 866–874

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Ermakova YG, Bilan DS, Matlashov ME, Mishina NM, Markvicheva KN, Subach OM, Subach FV, Bogeski I, Hoth M, Enikolopov G (2014) Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide. Nat Commun 5: 5222

Ewald JC, Reich S, Baumann S, Frommer WB, Zamboni N (2011) Engineering genetically encoded nanosensors for real-time in vivo measurements of citrate concentrations. PLoS One 6: e28245

Fehr M, Frommer WB, Lalonde S (2002) Visualization of maltose uptake in living yeast cells by fluorescent nanosensors. Proc Natl Acad Sci USA 99: 9846–9851

Gruenwald K, Holland JT, Stromberg V, Ahmad A, Watcharakichkorn D, Okumoto S (2012) Visualization of glutamine transporter activities in living cells using genetically encoded glutamine sensors. PLoS One 7: e38591

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Gutscher M, Pauleau A-L, Marty L, Brach T, Wabnitz GH, Samstag Y, Meyer AJ, Dick TP (2008) Real-time imaging of the intracellular glutathione redox potential. Nat Methods 5: 553–559

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Ho C-H, Frommer WB (2014) Fluorescent sensors for activity and regulation of the nitrate transceptor CHL1/NRT1. 1 and oligopeptide transporters. eLife 3: e01917

Horikawa K, Yamada Y, Matsuda T, Kobayashi K, Hashimoto M, Matsu-ura T, Miyawaki A, Michikawa T, Mikoshiba K, Nagai T (2010) Spontaneous network activity visualized by ultrasensitive Ca²⁺ indicators, yellow Cameleon-Nano. Nat Methods 7: 729–732

Hu H, Gu Y, Xu L, Zou Y, Wang A, Tao R, Chen X, Zhao Y, Yang Y (2017) A genetically encoded toolkit for tracking live-cell histidine dynamics in space and time. Sci Rep 7: 1–9

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Kaper T, Lager I, Looger LL, Chermak D, Frommer WB (2008) Fluorescence resonance energy transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Biotechnol Biofuels 1: 1–10

Kaper T, Looger LL, Takanaga H, Platten M, Steinman L, Frommer WB (2007) Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. PLoS Biol 5: e257

Kausar H, Ambrin G, Okla MK, Alamri SA, Soufan WH, Ibrahim EI, Abdel-Maksoud MA, Ahmad A (2021) FRET-based genetically encoded nanosensor for real-time monitoring of the flux of α-tocopherol in living cells. ACS omega 6: 9020–9027

Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, Kudla J, Schumacher K (2012) FRET-based genetically encoded sensors allow high-resolution live cell imaging of Ca²⁺ dynamics. The Plant journal : for cell and molecular biology 69: 181–92

Lager I, Fehr M, Frommer WB, Lalonde S (2003) Development of a fluorescent nanosensor for ribose. FEBS Lett 553: 85–89

Lager I, Looger LL, Hilpert M, Lalonde S, Frommer WB (2006) Conversion of a putative Agrobacterium sugar-binding protein into a FRET sensor with high selectivity for sucrose. J Biol Chem 281: 30875–30883

Lanquar V, Grossmann G, Vinkenborg JL, Merkx M, Thomine S, Frommer WB (2014) Dynamic imaging of cytosolic zinc in Arabidopsis roots combining FRET sensors and RootChip technology. New Phytol 202: 198–208

Lindenburg LH, Vinkenborg JL, Oortwijn J, Aper SJ, Merkx M (2013) MagFRET: the first genetically encoded fluorescent Mg²⁺ sensor. PloS one 8: e82009

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Mohsin M, Abdin MZ, Nischal L, Kardam H, Ahmad A (2013) Genetically encoded FRET-based nanosensor for in vivo measurement of leucine. Biosens Bioelectron 50: 72–77

Mohsin M, Ahmad A (2014) Genetically-encoded nanosensor for quantitative monitoring of methionine in bacterial and yeast cells. Biosens Bioelectron 59: 358–364

Morgan B, Van Laer K, Owusu TN, Ezeriņa D, Pastor-Flores D, Amponsah PS, Tursch A, Dick TP (2016) Real-time monitoring of basal H₂O₂ levels with peroxiredoxin-based probes. Nat Chem Biol 12: 437–443

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Okumoto S, Looger LL, Micheva KD, Reimer RJ, Smith SJ, Frommer WB (2005) Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors. Proc Natl Acad Sci USA 102: 8740–8745

Palmer AE, Giacomello M, Kortemme T, Hires SA, Lev-Ram V, Baker D, Tsien RY (2006) Ca²⁺ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol 13: 521–30

Park J, Chavez TM, Frommer W, Cheung LS (2020) Quantitative analysis of transporter activity biosensors. bioRxiv. doi: 10.1101/2020.09.03.282301

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Sadoine M., Ishikawa M., Kleist T., Wudick M.W., Nakamura M., Grossmann G., Frommer W.B.& Ho C.H. (2021) Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology. Plant Physiol. online. doi.org/10.1093/plphys/kiab353

Sahu A, Banerjee S, Raju AS, Chiou T-J, Garcia LR, Versaw WK (2020) Spatial profiles of phosphate in roots indicate developmental control of uptake, recycling, and sequestration. Plant Physiol 184: 2064–2077

San Martín A, Ceballo S, Ruminot I, Lerchundi R, Frommer WB, Barros LF (2013) A genetically encoded FRET lactate sensor and its use to detect the Warburg effect in single cancer cells. PloS one 8: e57712

Singh S, Sharma MP, Ahmad A (2020) Construction and characterization of protein-based cysteine nanosensor for the real time measurement of cysteine level in living cells. Int J Biol Macromol 143: 273–284

Singh S, Sharma MP, Alqarawi AA, Hashem A, Abd_Allah EF, Ahmad A (2019) Real-time optical detection of isoleucine in living cells through a genetically-encoded nanosensor. Sensors 20: 146

Tantama M, Yellen G (2014) Imaging changes in the cytosolic ATP-to-ADP ratio. Methods Enzymol 547: 355–371

Thestrup T, Litzlbauer J, Bartholomäus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y (2014) Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes. Nat Methods 11: 175–182

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Toyota M, Spencer D, Sawai-Toyota S, Jiaqi W, Zhang T, Koo AJ, Howe GA, Gilroy S (2018) Glutamate triggers long-distance, calcium-based plant defense signaling. Science 361: 1112–1115

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Waadt R, Hitomi K, Nishimura N, Hitomi C, Adams SR, Getzoff ED, Schroeder JI (2014) FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis. eLife 3: e01739

Waadt R, Köster P, Andrés Z, Waadt C, Bradamante G, Lampou K, Kudla J, Schumacher K (2020) Dual-reporting transcriptionally linked genetically encoded fluorescent indicators resolve the spatiotemporal coordination of cytosolic abscisic acid and second messenger dynamics in Arabidopsis. Plant Cell 32: 2582–2601

Yaginuma H, Kawai S, Tabata KV, Tomiyama K, Kakizuka A, Komatsuzaki T, Noji H, Imamura H (2014) Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging. Sci Rep 4: 1–7

Yang H, Bogner M, Stierhof Y-D, Ludewig U (2010) H⁺-independent glutamine transport in plant root tips. PLoS One 5: e8917

Yang Y, Liu N, He Y, Liu Y, Ge L, Zou L, Song S, Xiong W, Liu X (2018) Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP. Nature Communications 9: 1504

Zarowny L, Aggarwal A, Rutten VM, Kolb I, Project G, Patel R, Huang H-Y, Chang Y-F, Phan T, Kanyo R (2020) Bright and high-performance genetically encoded Ca²⁺ indicator based on mNeonGreen fluorescent protein. ACS sens 5: 1959–1968

Zhang C, Ye B-C (2014) A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell. Biosens Bioelectron 54: 15–19

Zhang WH, Herde MK, Mitchell JA, Whitfield JH, Wulff AB, Vongsouthi V, Sanchez-Romero I, Gulakova PE, Minge D, Breithausen B (2018) Monitoring hippocampal glycine with the computationally designed optical sensor GlyFS. Nat Chem Biol 14: 861–869

Zhao Y, Araki S, Wu J, Teramoto T, Chang Y-F, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T (2011) An expanded palette of genetically encoded Ca²⁺ indicators. Science 333: 1888–1891